Isolating single stranded DNA using a microfluidic dialysis device

Building a Modular Single-Stranded DNA Production Device Using Multi-Copy Single-Stranded DNA

An integrated optics microfluidic device for detecting single DNA molecules.

A fluorescence-based integrated optics microfluidic device is presented, capable of detecting single DNA molecules in a high throughput and reproducible manner. The device integrates microfluidics for DNA stretching with two optical elements for single molecule detection (SMD): a plano-aspheric refractive lens for fluorescence excitation (illuminator) and a solid parabolic reflective mirror for...

Developing a Device for Automated Peritoneal Dialysis

Background: Peritoneal dialysis (PD) is an efficient alternative for hemodialysis. Removal of waste metabolites and excess water from the blood circulation are the two main tasks done during dialysis. These are done by using a filter; in PD, the naturally semi-permeable peritoneal membrane acts as the filter. Automated PD machines control intelligently the operations to enhance the efficiency a...

Direct observation of single flexible polymers using single stranded DNA().

Over the last 15 years, double stranded DNA (dsDNA) has been used as a model polymeric system for nearly all single polymer dynamics studies. However, dsDNA is a semiflexible polymer with markedly different molecular properties compared to flexible chains, including synthetic organic polymers. In this work, we report a new system for single polymer studies of flexible chains based on single str...

Fluorescent single-stranded DNA-binding protein from Plasmodium falciparum as a biosensor for single-stranded DNA

Single-stranded DNA (ssDNA) is a product of many cellular processes that involve double-stranded DNA, for example during DNA replication and repair, and is formed transiently in many others. Measurement of ssDNA formation is fundamental for understanding many such processes. The availability of a fluorescent biosensor for the determination of single-stranded DNA provides an important route to a...